Norovirus fusion proteins and VLPS comprising norovirus fusion proteins
AssigneeMedicago Inc.
InventorsPierre-Olivier Lavoie, Marc-Andre D'Aoust
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Abstract
Nucleic acids encoding norovirus VP1 fusion proteins and VLPs comprising the norovirus VP1 fusion proteins are provided. Methods for norovirus VP1 fusion protein and norovirus VLP production in plants are also described. The VP1 fusion protein comprises, a first sequence encoding an S domain derived from a first norovirus strain, and a second sequence encoding a P domain derived from a second norovirus strain.
Claims22
A norovirus VP1 fusion protein comprising, an S domain derived from a first norovirus strain fused to a P domain derived from a second norovirus strain,
- wherein:
- the first norovirus strain is selected from norovirus genotype GI.1 and the second norovirus strain is selected from norovirus genotypes GI.2, GI.3, GII.4, GII.12, GII.13, and GII.17:
- the first norovirus strain is selected from norovirus genotype GI.5 and the second norovirus strain is norovirus genotype GII.4;
- the first norovirus strain is selected from norovirus genotype GII.1 and the second norovirus strain is selected from norovirus genotypes GI.3, GII.4, and GII.17;
- the first norovirus strain is selected from norovirus genotype GII.12 and the second norovirus strain is selected from norovirus genotypes GI.1, GI.2, GII.1, GII.2, GII.3, GII.4, GII.5, GII.7, GII.13, GII.14, GII.17 and GII.21;
- or
- the first norovirus strain is selected from norovirus genotype GII.14 and the second norovirus strain is norovirus genotype GII.4.
A nucleic acid molecule encoding a norovirus VP1 fusion protein, the nucleic acid molecule comprising a first sequence encoding an S domain derived from a first norovirus strain and a second sequence encoding a P domain derived from a second norovirus strain, the first and second sequence under the control of a promoter that is active in plants, wherein:
- the first norovirus strain is selected from norovirus genotype GI.1 and the second norovirus strain is selected from norovirus genotypes GI.2, GI.3, GII.4, GII.12, GII.13, and GII.17;
- the first norovirus strain is selected from norovirus genotype GI.5 and the second norovirus strain is norovirus genotype GII.4;
- the first norovirus strain is selected from norovirus genotype GII.1 and the second norovirus strain is selected from norovirus genotypes GI.3, GII.4, and GII.17;
- the first norovirus strain is selected from norovirus genotype GII.12 and the second norovirus strain is selected from norovirus genotypes GI.1, GI.2, GII.1, GII.2, GII.3, GII.4, GII.5, GII.7, GII.13, GII.14, GII.17 and GII.21;
- or
- the first norovirus strain is selected from norovirus genotype GI1.14 and the second norovirus strain is norovirus genotype GII.4.
Dependencies are inferred from the claim text.