Method for improving rice yield by jointly knocking out ABA receptor PYL family genes and use thereof

A method for producing transgenic rice plant cells, a transgenic rice plant tissue, transgenic rice plant, or transgenic seed or grain derived therefrom, comprising the steps:

• (i) reducing or eliminating rice endogenous Pyrabactin Resistant 1-Like 1 (PYL1) and Pyrabactin Resistant 1-Like 6 (PYL6) gene expression by CRISPR technology to reduce or eliminate expression of the rice endogenous PYL1 and PYL6 genes in rice cells, wherein the CRISPR technology comprises transforming said rice cells with a recombinant CRISPR/Cas9 vector targeted to said rice endogenous PYL1 and PYL6 genes to reduce or eliminate expression of said rice endogenous PYL1 and PYL6 genes thereby producing transformed rice cells;
• (ii) regenerating said transformed rice cells of step (i) into a regenerated transformed rice plant and performing a trait testing on the regenerated transformed rice plant, wherein the trait is selected from the group consisting of plant height, plant yield and plant biomass;
• and
• (iii) based on the results of the trait testing from step (ii), selecting a regenerated transformed rice plant with an improved plant trait characteristic, wherein the improved plant trait characteristic comprises increased plant height, increased plant yield or increased plant biomass, and wherein the transgenic rice plant cells, said transgenic rice plant tissue, transgenic rice plant, or transgenic rice seed or rice grain derived therefrom is obtained from the selected regenerated transformed rice plant with the improved plant trait characteristic.