Method for preparation of derivatives of gram-positive bacteria surface capsular polysaccharide

A gram-positive bacteria cell wall capsular polysaccharide compound, comprising one or more of a disaccharide, a trisaccharide, a tetrasaccharide, a pentaose, a hexaose, a heptaose, an octaose, a nonose and a decose:

• wherein a structure of the decose is shown in formula I:
• wherein R6 is an aminolink [—(CH2)n—N—Y1Y2, n=1 to 10], and is used to be linked with a protein, n represents that the aminolink has different carbon chain lengths, Y1 and Y2 are protecting groups for amino, and independently selected from H, benzyl (Bn), or benzyloxycarbonyl (Cbz);
• wherein R2, R3, R4 and R5 groups are independently selected from H, acetyl (Ac), benzoyl (Bz), pivalyl (Piv), chloracetyl (ClAc), levulinic acyl (Lev), allyloxycarbonyl (Alloc), benzyl (Bn), 2-menaphthyl (Nap), allyl (All), benzylidene acetal or isopropylidene ketal;
• wherein R7 and R12 groups are independently selected from H, acetyl (Ac), benzoyl (Bz), pivalyl (Piv), chloracetyl (ClAc), levulinic acyl (Lev) or allyloxycarbonyl (Alloc), and R8 is H, benzyl (Bn) or allyl (All);
• and wherein R13 and R14 are independently selected from H, benzyl (Bn), 2-menaphthyl (Nap), allyl (All), benzylidene acetal or isopropylidene ketal;
• wherein the disaccharide is shown in the following formula:
• wherein R2, R3, R4, R5, R6, R7 and R8 refer to formula I;
• the trisaccharide is shown in the following formula:
• wherein R12, R13, R14, R6, R7 and R8 refer to formula I;
• the tetrasaccharide is shown in the following formula:
• wherein R2, R3, R4, R5, R6, R7, R8, R12, R13 and R14 refer to formula I;
• the pentaose is shown in the following formula:
• wherein R6, R7, R8, R12, R13 and R14 refer to formula I;
• the hexaose is shown in the following formula:
• wherein R2, R3, R4, R5, R12, R13, R14, R6, R7 and R8 refer to formula I;
• the heptaose is shown in the following formula,
• wherein R12, R13, R14, R6, R7, R8 and R9 refer to formula I;
• and R9 groups is H, acetyl (Ac), benzoyl (Bz), pivalyl (Piv), chloroacetyl (ClAc), levulinic acyl (Lev), allyloxycarbonyl (Alloc), benzyl (Bn), 2-menaphthyl (Nap), p-Methoxybenzyl (pMBn), allyl (All), benzylidene acetal or isopropylidene ketal;
• the octaose is shown in the following formula:
• wherein R2, R3, R4, R5, R12, R13, R14, R6, R7 and R8 refer to formula I;
• and
• the nonose is shown in the following formula:
• wherein R12, R13, R14, R6, R7 and R8 refer to formula I.