Methods and compositions related to improved nitrogen utilization efficiency in tobacco
AssigneeAltria Client Services LLC
InventorsJesse Frederick, Chengalrayan Kudithipudi, Dongmei Xu
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Abstract
The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.
Claims19
A method of producing a tobacco plant comprising an enhanced nitrogen utilization efficiency (NUE) trait, said method comprising:
- a. providing a first population of tobacco plants comprising at least one tobacco plant comprising an enhanced NUE trait;
- b. obtaining nucleic acids from said first population of tobacco plants;
- c. genotyping said nucleic acids from said first population of tobacco plants via a molecular assay for the presence of one or more molecular markers located within 10 cM of a SNP marker comprising SEQ ID NO:
- 58;
- d. selecting a tobacco plant comprising said one or more molecular markers and a favorable NUE allele at SEQ ID NO:
- 58;
- e. crossing said tobacco plant selected in step (d) with a second tobacco plant;
- and
- f. obtaining progeny seed from the cross of step (e) wherein a plant grown from said progeny seed comprises an enhanced NUE trait and said SNP marker.
A method of introgressing an enhanced NUE trait into a tobacco variety, said method comprising the steps of:
- a. crossing a first tobacco variety comprising an enhanced nitrogen utilization efficiency trait with a second tobacco variety lacking said enhanced nitrogen utilization efficiency trait;
- b. obtaining progeny seed from the cross of step (a);
- c. genotyping at least one of said progeny seed obtained in step (b) via a molecular assay for a molecular marker linked within 10 cM to a SNP marker comprising SEQ ID NO:
- 58;
- and
- d. selecting a progeny seed comprising at least one favorable NUE allele at said SNP marker and further comprising said enhanced nitrogen utilization efficiency trait.
Dependencies are inferred from the claim text.