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US 11,602,107 B2Granted
Graft-mediated hybridisation of monocotyledonous plants
AssigneeCambridge Enterprise Limited
InventorsGregory Reeves, Julian Hibberd
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Abstract
The invention relates to the production of graft-mediated hybrid monocotyledonous plants. Methods for the production of such plants are disclosed herein.
Claims24
A method of producing a hybrid monocot tissue or plant, comprising:
- (a) providing a first tissue comprising:
- (i) mesocotyl and radicle tissue;
- or
- (ii) mesocotyl and plumule tissue
- of a first monocot plant, wherein said first tissue comprises in its genome a first marker;
- (b) providing a second tissue comprising:
- (i) mesocotyl and radicle tissue;
- or
- (ii) mesocotyl and plumule tissue
- of a second, different monocot plant, wherein said second tissue comprises in its genome a second, different marker;
- (c) placing said first tissue in contact with said second tissue;
- (d) allowing fusion of the first and second tissues such that a graft junction forms, wherein said graft junction comprises at least one hybrid cell comprising said first and second markers;
- (e) selecting said at least one hybrid cell based on the presence of said first and second markers;
- and
- (f) regenerating a hybrid monocot tissue or plant from said at least one hybrid cell.
12 more elements
Dependencies are inferred from the claim text.