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US 11,600,361 B2Granted
Nucleic acid sequence assembly
AssigneeDovetail Genomics, LLC
InventorsNicholas H. Putnam, Jonathan C. Stites, Brandon J. Rice
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Abstract
Disclosed herein are compositions, systems and methods related to sequence assembly, such as nucleic acid sequence assembly of single reads and contigs into larger contigs and scaffolds through the use of read pair sequence information, such as read pair information indicative of nucleic acid sequence phase or physical linkage.
Claims19
A method for nucleic acid sequence data assembly, comprising:
- (a) obtaining a set of paired-end reads obtained by digesting sample DNA to generate internal double strand breaks within the DNA, allowing the double strand breaks to re-ligate randomly to form a plurality of junctions, and sequencing across the plurality of junctions;
- (b) obtaining standard paired-end read distance frequency data;
- (c) obtaining grouped contig sequences;
- and
- (d) scaffolding the grouped contig sequences such that read-pair distance frequency data for read pairs that map to separate contigs approximates the standard paired-end read distance frequency data,
- thereby assembling nucleic acid sequence data of the nucleic acid.
3 more elements
Dependencies are inferred from the claim text.