Process for diagnosing chronic inflammatory intestinal diseases
AssigneesUniversite De Nantes, Chu De Nantes—Centre Hospitalier Universitaire De Nantes, Inserm (Institut National De La Sante Et De La Recherche Medicale)
InventorsJérémy Brégeon, Michel Neunlist, Emmanuel Coron
Life of this patent
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Abstract
Some embodiments are directed to a process for quantifying changes in the intestinal mucosa caused by a chronic inflammatory intestinal disease in individuals, an ex vivo process for diagnosing a chronic inflammatory intestinal disease in individuals, and an ex vivo process for the differential diagnosis of Crohn's disease versus ulcerative colitis in individuals.
Claims19
An ex vivo process for quantifying the modifications of the intestinal mucosa induced by a chronic inflammatory bowel disease in an individual, the process comprising:
- 1) Measuring, on a confocal endomicroscopic image of a digestive mucosa of an individual, following crypt well profile (PPC) parameters:
- a) Fluorescence leakage of the mucosa (FL),
- b) Perimeter of opening of the crypt well (PO),
- c) Sphericity of the crypt well (Spher),
- d) Roundness of the crypt well (Round),
- e) Feret diameter of the crypt well (Feret),
- f) Crypt well elongation factor (EF),
- g) Ratio of axes of opening of the crypt well (Ma/ma),
- h) Crypt density per field (Dens);
- and
- 2) quantifying modifications of the parameters a) to h) compared with corresponding parameters of a healthy individual.
An ex vivo process for diagnosing a chronic inflammatory bowel disease in an individual, the process comprising:
- 1) Measuring, on a confocal endomicroscopic image of digestive mucosa of an individual, following parameters:
- a) Fluorescence leakage of the mucosa (FL),
- b) Perimeter of opening of the crypt well (PO),
- c) Sphericity of a crypt well (Spher),
- d) Roundness of the crypt well (Round),
- e) Feret diameter of the crypt well (Feret),
- f) Crypt well elongation factor (EF),
- g) Ratio of axes of opening of the crypt well (Ma/ma),
- h) Crypt density per field (Dens);
- 2) Selecting values described as “higher extremes”, above thresholds defined below for each parameter:
- PO>474.62±103.10 μm,
- Spher>98.96±0.56%,
- Round>68.43±14.41%,
- Feret>169.88±51.08 μm,
- EF>1.745±0.266,
- Ma/ma>1.653±0.247,
- Dens>0.2837±0.0505;
- 3) Comparing, for each parameter, the mean of the “higher extreme” values and the mean value of FL to a pathological threshold, the pathological threshold being defined, for each parameter, in a following way:
- FL>10.0±3.6 LUT.sec−1,
- PO>666.40±103.10 μm,
- Spher>99.32±0.56%,
- Round>73.00±14.41%,
- Feret>186.11±51.08 μm,
- EF>1.87±0.266,
- Ma/ma>1.90±0.247,
- Dens>0.39±0.0505;
- 4) Assigning following scores:
- if the mean of the “higher extreme” values of each parameter PO, Spher, Round, Feret, EF, Ma/ma and Dens, or the mean value of FL, is less than the pathological threshold, the score of 0 is assigned to a corresponding parameter,
- if the mean of the “higher extreme” values of each parameter PO, Spher, Round, Feret, EF, Ma/ma and Dens, or the mean value of FL, is greater than the pathological threshold, a score of 1 is assigned for FL, PO, Round, Feret and Dens, and a score of 2 is assigned for Spher, EF and Ma/ma, and
- 5) Multiplying the sum of the scores by the sum of the number of values of each parameter,
- in which a total greater than 204±110 defines the individual as suffering from a chronic inflammatory bowel disease.
Dependencies are inferred from the claim text.