Optical device, phase plate, and image forming method
AssigneeSysmex Corporation
InventorsMasaya Okada, Shigeki Iwanaga
Life of this patent
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Abstract
An optical device comprises a shared phase modulation mask configured to impart a first phase modulation to light of a first wavelength, and imparts a second phase modulation to light of a second wavelength, an irradiation optical system configured to cause the light of the first wavelength and the light of the second wavelength to enter the same incident region in the phase modulation mask, and a light collecting optical system configured to collect the light of the first phase-modulated first wavelength and the light of the second phase-modulated second wavelength to form an image corresponding to a point spread function.
Claims17
A method for forming images according to a point spread function, the method comprising:
- directing light of a first wavelength having a center wavelength at λ1 and light of a second wavelength having a center wavelength at λ2 to a phase plate to modulate the phases of the light, and
- collecting phase-modulated light of the first wavelength and phase-modulated light of the second wavelength to form images respectively for each light of wavelength,
- wherein a thickness T3 of the phase plate is set between a first thickness T1 and a second thickness T2, each represented by the following equations:
- T1=λ1×θ/{2π(n2−n1)}
- T2=λ2×θ/{2π(n2−n1)}
- T3={(T1×a)+(T2×b)}/(a+b)
- wherein:
- a and b are both positive real numbers,
- T1 is an optimal thickness of a phase plate to form an image of light at the first wavelength,
- T2 is an optimal thickness of a phase plate to form an image of light at the second wavelength,
- n1 is a refractive index of air,
- n2 is a refractive index of the phase plate,
- θ is a phase shift amount, and
- wherein T3 is set within a range where (a, b)=(2, 8) to (8, 2).
A method for forming images according to a point spread function, the method comprising:
- irradiating a sample with light of a first wavelength and light of a second wavelength;
- directing a first fluorescent light corresponding to the first wavelength and a second fluorescent light corresponding to the second wavelength from the sample to a phase plate to modulate the phases of the first and second fluorescent light, the phase plate made of a transparent material and being transparent and comprising a geometry hybrid of a plurality of first phase modulation regions for the first fluorescent light and a plurality of second phase modulation regions for the second fluorescent light, wherein the first phase modulation regions and the second phase modulation regions are arranged in a mosaic, and the first and second fluorescent lights are directed to pass the first phase modulation regions and the second phase modulation regions at the same time;
- collecting phase-modulated first fluorescent light and phase-modulated second fluorescent light to an imaging device
- obtaining a first image of the phase-modulated first fluorescent light by the imaging device;
- and
- obtaining a second image of the phase-modulated second fluorescent light by the imaging device.
A method for forming images according to a point spread function, the method comprising:
- irradiating a sample with light of a first wavelength and light of a second wavelength;
- configuring a phase modulation mask to represent on its surface a hybrid phase modulation pattern of a plurality of first phase modulation regions for the light of the first wavelength and a plurality of second phase modulation regions for the light of a second wavelength;
- directing a first fluorescent light corresponding to the first wavelength and a second fluorescent light corresponding to the second wavelength from the sample to the phase modulation mask to modulate the phases of the first and second fluorescent light with the hybrid phase modulation pattern, wherein the plurality of first phase modulation regions and the plurality of second phase modulation regions are arranged in a mosaic, and wherein the first and second fluorescent lights are directed to pass the first phase modulation regions and the second phase modulation regions at the same time;
- collecting phase-modulated first fluorescent light and phase-modulated second fluorescent light to an imaging device;
- obtaining a first image of the phase-modulated first fluorescent light by the imaging device;
- and
- obtaining a second image of the phase-modulated second fluorescent light by the imaging device
- wherein the plurality of first phase modulation regions and the plurality of second phase modulation regions are arranged in a mosaic.
Dependencies are inferred from the claim text.