Multi-channel line scanner for fast large field and confocal imaging
AssigneeCalifornia Institute of Technology
InventorLong Cai
Life of this patent
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Abstract
A multi-channel line microscope for single molecule Fluorescence In Situ Hybridization (FISH) imaging of a sample. A microscope stage moves a sample across two or more reflected excitation lines positioned relative to each other so that each excitation line excites a spatially distinct horizontal line in the image plane of the sample. A sample is imaged by moving the microscope stage across two or more reflected excitation lines positioned relative to each other so that each excitation line excites a spatially distinct horizontal line in the image plane of the sample. The apparatus and methods of use are suitable for a broad range of applications.
Claims12
A method of fluorescence imaging of single molecules in a sample, comprising:
- placing the sample on a microscope stage, wherein the sample comprises one or more targets and one or more probes, wherein the probes are capable of emitting visually detectable signals, and wherein each individual probe is hybridized to a unique target in the sample;
- moving the microscope stage in a single direction linearly or circularly;
- exciting one or more probes with excitation lines, wherein two or more excitation lines are positioned relative to each other such that each excitation line excites a spatially distinct horizontal line in an image plane of the sample, and wherein two or more excitation lines are positioned about 0.5 μm to about 100 μm apart from each other;
- and
- detecting one or more signals emitted by the one or more probes.
Dependencies are inferred from the claim text.