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US 11,598,792 B2Granted
Voltage indicators
AssigneeHoward Hughes Medical Institute
InventorsEric R. Schreiter, Ahmed Abdelfattah
Life of this patent
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Abstract
A voltage indicator includes a polypeptide sequence comprising a voltage-sensitive opsin domain and a capture protein domain arranged and disposed to capture a fluorescent dye ligand. When the fluorescent dye ligand is captured and the voltage indicator is bound to a cell membrane, an increase in voltage across the cell membrane causes an increase in fluorescent emission.
Claims18
A voltage indicator, comprising:
- a voltage-sensitive microbial rhodopsin domain comprising the amino acid sequence of SEQ ID NO:
- 9 with an amino acid mutation at one or more of residue 81, 92, and 199;
- and a capture protein that covalently or noncovalently binds a fluorescent dye ligand that is (i) a fluorescent protein;
- or (ii) a fluorescent dye, wherein the capture protein is provided together with the voltage-sensitive microbial rhodopsin domain in a fusion protein.
1 more element
A voltage indicator, comprising an amino acid sequence selected from the group of amino acid sequences of SEQ ID NOS:
- 2, 4, 6, and 8.
A voltage indicator, comprising the amino acid sequence of SEQ ID NO:
- 20.
Dependencies are inferred from the claim text.