Method for predicting the risk of incidence of chronic kidney disease
AssigneeSphingotec GmbH
InventorsAndreas Bergmann, Olle Melander
Life of this patent
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Abstract
The present invention relates to means and methods suitable for risk prediction of chronic kidney disease (CKD) using Pro-Enkephalin or fragments thereof as biomarker. The risk prediction methods of the invention are intended for healthy subjects and for subjects suffering from diseases such as hypertension, cardiovascular diseases and events, diabetes, metabolic syndrome, obesity, or autoimmune diseases. Subject matter of the invention is also a method of predicting the worsening or improvement of kidney function or dysfunction in healthy and diseased individuals.
Claims14
A method comprising:
- measuring the level of a Pro Enkephalin protein comprising the amino acid sequence of SEQ ID NO:1 or fragments thereof in a sample of bodily fluid obtained from a subject,
- wherein, before obtaining said sample,
- a prior sample of bodily fluid of said subject exhibited a level of a Pro Enkephalin protein comprising the amino acid sequence of SEQ ID NO:1 or fragments thereof above 30 pmol/L and said subject was subjected to therapeutic measures to prevent development of chronic kidney disease,
- wherein said fragments of Pro Enkephalin comprise the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, or SEQ ID NO:
- 10, and
- wherein, at the time of obtainment of said prior sample, the subject was a
- (i) healthy subject, or
- (ii) diseased subject that did not have chronic kidney disease and that had an estimated glomerular filtration rate of greater than 60 ml/min/1.73 m2 for >3 months.
A method for preparing a sample comprising:
- obtaining a sample of bodily fluid from a subject;
- and
- adding to said sample, a binder that binds to a Pro Enkephalin protein comprising the amino acid sequence of SEQ ID NO:1, or one or more fragments thereof,
- wherein said one or more fragments comprise the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10 or SEQ ID NO:
- 11,
- wherein, at the time of obtaining said sample, the subject is a
- (i) healthy subject, or
- (ii) diseased subject that does not have chronic kidney disease, and that has had an estimated glomerular filtration rate of greater than 60 ml/min/1.73 m2 for >3 months, and
- wherein in said sample, said binder is bound to the Pro Enkephalin or one or more of said fragments at a level above 30 pmol/L.
Dependencies are inferred from the claim text.