Detection of cancer biomarkers using nanoparticles
AssigneeThe University of Manchester
InventorsKostas Kostarelos, Marilena Hadjidemetriou
Life of this patent
Estimating the term…
Abstract
Methods for identifying and detecting potential disease specific biomarkers from biofluids. The methods involve in vivo administration of nanoparticles to a subject in a diseased state or incubating nanoparticles in a biofluid sample taken from a subject in a diseased state and analysis of the biomolecule corona formed on said nanoparticles. The methods distinguish between a healthy and diseased state in a subject, such as, for example, the presence of a tumor in a human subject.
Claims13
A method of discovering unique disease-specific biomarker not previously known to be associated with the disease, wherein the method comprises:
- a. incubating a plurality of nanoparticles in a biofluid sample taken from a subject suffering from the disease and allowing a biomolecule corona to form on the surface of the nanoparticles;
- b. isolating the nanoparticles and surface-bound biomolecule corona;
- c. analyzing the isolated nanoparticles and surface-bound biomolecule corona by mass spectrometry and generating a mass spectrum;
- d. comparing the mass spectrum with a mass spectrum generated from a biofluid sample from a subject not suffering from the disease, which serves as a suitable non-diseased control reference, and identifying differences;
- and
- e. discovering a unique disease-specific biomarker not previously known to be associated with the disease based on the differences.
Dependencies are inferred from the claim text.