Method and devices for rapid diagnosis of foot-and-mouth disease

A method for simultaneously detecting and differentiating between antibodies of interest comprising antibodies to FMDV structural proteins and antibodies to FMDV non-structural proteins in a sample, comprising:

• a. obtaining a fluid test sample to be assayed for said antibodies of interest;
• b. supplying a rapid measurement device comprising a membrane strip with a proximal end containing a labeled binding partner and a labeled control reagent, and a distal end containing a zone having at least one immobilized capture reagent specific for an FMDV structural protein comprising at least one of VP1, VP2, VP3 or VP4, and a second zone having at least one immobilized capture reagent specific for an FMDV non-structural protein comprising at least one of Lb, 2B, 2C, 3A, 3AB, or 3ABC, and a third band of immobilized control capture reagent wherein said fluid test sample moves from said proximal end to said distal end by capillary action;
• c. contacting said membrane strip with said fluid test sample;
• d. maintaining said membrane strip under conditions which are sufficient to allow said antibodies of interest to bind to said labeled binding partner, forming a specific binding complex, and to allow fluid to transport said specific binding complex and said labeled control reagent in said fluid by capillary action through said membrane strip to said immobilized capture reagent specific for an FMDV structural protein and said adjacent immobilized capture reagent specific for an FMDV non-structural protein and to said immobilized control capture reagent;
• e. maintaining said membrane strip under conditions which are sufficient to allow said specific binding complex in said fluid to bind to at least one of said immobilized capture reagent specific for an FMDV structural protein and/or to bind to at least one of said immobilized capture reagent specific for an FMDV non-structural protein and said labeled control reagent to bind to said immobilized control capture reagent;
• f. detecting an amount of said antibodies of interest in said fluid test sample and an amount of internal control signal, as indicated by an intensity of a signal producing system;
• and
• g. differentiating between said antibodies of interest.