Method and products for the diagnosis of a seafood allergy
AssigneesEUROIMMUN Medizinische Labordiagnostika AG, Hospital Clinic De Barcelona, Institut D'Investigacions BiomèDiques August Pi I Sunyer
InventorsWaltraud Suer, Marco Klinge, Alf Weimann, Stefanie Rohwer, Henning Seismann, Mariona Pascal Capdevila
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Abstract
A diagnostically useful carrier includes a means for specifically capturing an antibody to a polypeptide from the group including squid MLC1 or squid MLC2 or a variant thereof in a sample from a subject. A method includes the step detecting in a sample from a subject the presence or absence of an antibody to squid MLC1 or squid MLC2. The polypeptide or the carrier or a polypeptide binding specifically to an IgE antibody from the sample of a patient to squid MLC1 or squid MLC2 are useful for the manufacture of a diagnostic kit, preferably for the diagnosis of allergy.
Claims20
A solid phase, comprising:
- a recombinant and/or purified polypeptide of squid myosin light chain 2 (MLC2) or an immunoreactive fragment thereof, the immunoreactive fragment comprising at least one of SEQ ID NO:
- 10, 30, 31, 42, 43, or 51 for specifically capturing an antibody to at least one of MLC2 in a sample from a subject, wherein the polypeptide of squid MLC2 or the fragment thereof optionally comprises at least one affinity tag and/or at least one linker.
A method, comprising:
- contacting a sample from a subject to a solid phase having immobilized thereon a recombinant and/or purified polypeptide of squid myosin light chain 2 (MLC2) or an immunoreactive fragment thereof, the immunoreactive fragment comprising at least one of SEQ ID NO:
- 10, 30, 31, 42, 43, or 51, wherein the polypeptide of squid MLC2 or the fragment thereof optionally comprises at least one affinity tag and/or at least one linker;
- and
- detecting an antibody that binds to the MLC2 polypeptide or immunoreactive fragment thereof.
A method, comprising:
- contacting a medical or diagnostic device comprising a polypeptide with a solution comprising an antibody,
- wherein the polypeptide is a recombinant and/or purified polypeptide comprising squid myosin light chain 2 (MLC2) or an immunoreactive fragment thereof, the immunoreactive fragment comprising at least one of SEQ ID NO:
- 10, 30, 31, 42, 43, or 51, wherein the polypeptide of squid MLC2 or the fragment thereof optionally comprises at least one affinity tag and/or at least one linker.
Dependencies are inferred from the claim text.