Compositions and methods for passive optical barcoding for multiplexed assays
AssigneeSlingshot Biosciences, Inc.
InventorsJeffrey Kim, Anh Tuan Nguyen, Brandon Miller
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Abstract
Compositions comprising multiple hydrogel particles having substantially the same diameter, but with each subgrouping of particles from the multiple hydrogel particles having different associated values for one or more passive optical properties that can be deconvoluted using cytometric instrumentation. Each hydrogel particle from the multiple hydrogel particles can be functionalized with a different biochemical or chemical target from a set of targets. A method of preparing hydrogel particles includes forming droplets and polymerizing the droplets, with optional functionalization.
Claims21
A method of performing a multiplexed assay of a sample comprising chemical, cellular, or biochemical targets, the method comprising:
- combining the sample and a plurality of hydrogel particles having passive optical properties and being functionalized, thereby causing a reaction and formation of an optically encoded hydrogel particle-target combination having at least one hydrogel particle from the plurality of hydrogel particles bound to at least one of the targets;
- measuring, with a cytometric device, the passive optical properties of the at least one hydrogel particle of the optically encoded hydrogel particle-target combination;
- deconvoluting the plurality of bound hydrogel particles within the sample based on the passive optical properties of the plurality of hydrogel particles;
- and
- identifying an assay result for the sample based on the deconvolution.
Dependencies are inferred from the claim text.