Platform for native liquid chromatography-mass spectrometry
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsShunhai Wang, Yuetian Yan
Life of this patent
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Abstract
Disclosed are native liquid chromatography-mass spectrometry systems and methods of use. A native liquid chromatography-mass spectrometry system can include a liquid chromatography system capable of separating a sample; and an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter and a system for modifying a desolvation gas and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions.
Claims20
A native liquid chromatography-mass spectrometry system, comprising:
- a liquid chromatography system capable of separating a sample under non-denaturing conditions;
- and
- an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter, a system for modifying a desolvation gas, and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions,
- wherein the system for modifying a desolvation gas comprises a container having a cap and comprising a modifier selected from the group consisting of isopropanol, acetonitrile, and ammonia in acetonitrile, wherein the cap has an inlet line port and an outlet line port;
- a sheath gas inlet line for providing a sheath gas to the inlet line port and a modified desolvation gas outlet line capable of connecting the modified desolvation gas outlet line port to the multi-nozzle electrospray ionization emitter.
A method of characterizing a protein in a sample, comprising:
- supplying the sample to a liquid chromatography system capable of sample separation under non-denaturing conditions, the liquid chromatography system comprising a size exclusion chromatography system (SEC) column or an ion exchange chromatography (IEX) column;
- and
- analyzing the sample by use of an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter, a system for modifying a desolvation gas, and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions to identify the components of the protein to characterize the protein,
- wherein the method does not require deglycosylation of the protein in the sample prior to supplying the sample to the liquid chromatography system, and the system for modifying a desolvation gas comprises a container having a cap and comprising a modifier selected from the group consisting of isopropanol, acetonitrile, and ammonia in acetonitrile, wherein the cap has an inlet line port and an outlet line port;
- a sheath gas inlet line for providing a sheath gas to the inlet line port and a modified desolvation gas outlet line capable of connecting the modified desolvation gas outlet line port to the multi-nozzle electrospray ionization emitter.
Dependencies are inferred from the claim text.