Method of analysing an aqueous fluid using 2D-IR spectroscopy
AssigneeThe University of York
InventorsMatthew Baker, Neil T. Hunt, Samantha Rutherford, Gordon Hithell
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Abstract
A method of analysing an aqueous fluid comprising obtaining a 2D-IR spectrum of a sample of the aqueous fluid using a 2D-IR spectrometer configured to apply a sequence of IR pulses to the sample, wherein the sequence comprises a pump process followed by a probe pulse, where the pump process is a single pump pulse or a sequence of a first pump pulse and a second pump pulse, and a waiting time Tw between applying the single pump pulse or the second pump pulse and applying the probe pulse is from 150 to 350 fs.
Claims20
A method of analyzing an aqueous fluid, said method comprising:
- obtaining a 2D-IR spectrum of a sample of the aqueous fluid using a 2D-IR spectrometer configured to apply a sequence of IR pulses to the sample, and
- determining whether or not one or more proteins are present in the aqueous fluid by observing the amide I band of the one or more proteins,
- wherein the sequence comprises a pump process followed by a probe pulse, where the pump process is a single pump pulse or a sequence of a first pump pulse and a second pump pulse, and a waiting time Tw between applying the single pump pulse or the second pump pulse and applying the probe pulse is from 150 to 350 fs.
A method of analyzing an aqueous fluid, said method comprising:
- obtaining a 2D-IR spectrum of a sample of the aqueous fluid using a 2D-IR spectrometer configured to apply a sequence of IR pulses to the sample, the 2D-IR spectrum including peaks due to albumin and globulins, and
- determining a ratio of albumin to globulins present in the sample,
- wherein the sequence comprises a pump process followed by a probe pulse, where the pump process is a single pump pulse or a sequence of a first pump pulse and a second pump pulse, and a waiting time Tw between applying the single pump pulse or the second pump pulse and applying the probe pulse is from 150 to 350 fs.
A method of analyzing an aqueous fluid, said method comprising:
- obtaining a 2D-IR spectrum of a sample of the aqueous fluid using a 2D-IR spectrometer configured to apply a sequence of IR pulses to the sample, the 2D-IR spectrum including a peak due to albumin and a peak due to γ-globulins, and
- determining a ratio of albumin to γ-globulins by measuring a peak height of the albumin peak and a peak height of the γ-globulins peak and dividing the peak height of the albumin peak by the peak height of the γ-globulins peak multiplied by 1.8,
- wherein the sequence comprises a pump process followed by a probe pulse, where the pump process is a single pump pulse or a sequence of a first pump pulse and a second pump pulse, and a waiting time Tw between applying the single pump pulse or the second pump pulse and applying the probe pulse is from 150 to 350 fs.
Dependencies are inferred from the claim text.