Rapid selective detection of bacteria
AssigneeChapman University
InventorsKamaljit Kaur, Hanieh Hossein-Nejad-Ariani
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Abstract
A rapid assay for determining the presence of bacteria in a sample, such as a contaminated food sample, is disclosed. The assay comprises contacting the sample with a bacteria-specific ligand associated with a substrate, wherein bacteria present in the sample bind the ligand; contacting the bound bacteria with a detection agent; detecting the presence of bacteria in the sample by measuring the quantity of detection agent associated with the sample.
Claims10
An assay for determining the presence of Listeria bacteria in a sample comprising:
- contacting the sample with a Listeria bacteria-specific ligand non-covalently immobilized on a glass substrate, wherein Listeria bacteria present in the sample binds to the ligand, wherein the bacteria-specific ligand is Leucocin A (LeuA);
- contacting the bound Listeria bacteria with a detection agent, wherein the detection agent binds to the Listeria bacteria in the sample;
- and
- detecting the presence of Listeria bacteria in the sample by measuring the quantity of detection agent bound to Listeria bacteria in the sample.
An assay for determining the presence of Listeria bacteria in a sample comprising:
- contacting the sample with a Listeria bacteria-specific ligand immobilized on a polyvinylidene difluoride (PVDF) membrane, wherein Listeria bacteria present in the sample binds to the ligand, wherein the bacteria-specific ligand is Leucocin A (LeuA);
- contacting the bound Listeria bacteria with a detection agent, wherein the detection agent binds to the Listeria bacteria in the sample;
- and
- detecting the presence of Listeria bacteria in the sample by measuring the quantity of detection agent bound to Listeria bacteria in the sample.
Dependencies are inferred from the claim text.