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US 11,597,961 B2Granted
Rapid colorimetric diagnostic test for C. difficile
AssigneeQualitic Biotechnology LLC
InventorsDanae Rammos, Madhumita Baskaran, Pranav Dorbala, Allison Julie Wong
Life of this patent
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Abstract
In certain aspects, provided herein are methods and kits for detecting the presence of C. difficile in a biological sample. Also provided herein are methods of selecting of identifying a subject that would benefit from C. difficile treatment.
Claims15
A method of detecting Clostridioides difficile (C. difficilel), comprising:
- (a) applying a fecal sample to a filter paper medium loaded with glutamate, and an oxidizing agent selected from the group consisting of nicotinamide-adenine dinucleotide (NAD+), and nicotinamide-adenine dinucleotide phosphate (NADP+), wherein the filter paper medium comprises a window for direct application of the fecal sample;
- (b) applying a solution comprising an electron transfer agent and a tetrazolium dye precursor to the filter paper medium;
- and
- (c) detecting C. difficile in the fecal sample colorimetrically based on the activity of glutamate dehydrogenase enzyme of the C. difficile present in the fecal sample.
1 more element
A kit for detecting Clostridioides difficile (C. difficile) in a fecal sample, the kit comprising:
- (a) a filter paper medium loaded with glutamate, and an oxidizing agent selected from the group consisting of nicotinamide-adenine dinucleotide (NAD+), and nicotinamide-adenine dinucleotide phosphate (NADP+), wherein the filter paper medium comprises a window for direct application of a fecal sample;
- and
- (b) a solution comprising an electron transfer agent and a tetrazolium dye precursor;
- wherein the kit is configured to detect the presence of C. difficile in the fecal sample colorimetrically based on the activity of glutamate dehydrogenase enzyme of the C. difficile present in the fecal sample.
1 more element
Dependencies are inferred from the claim text.