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US 11,597,957 B2Granted
Using dissolved oxygen to inhibit lactic acid production during propagation of yeast and/or hydrolysis of lignocellulosic biomass
AssigneePOET Research, Inc.
InventorZachary J Karl
Life of this patent
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Abstract
Embodiments of the present disclosure involve systems and methods that inhibit the production of lactic acid during propagation of yeast and/or during hydrolysis of cellulose by including a sufficient amount of dissolved oxygen.
Claims20
A method of treating lignocellulosic biomass, wherein the method comprises:
- providing an aqueous slurry comprising the lignocellulosic biomass and one or more enzymes, wherein the lignocellulosic biomass comprises cellulose, and wherein the one or more enzymes can convert the cellulose into an oligosaccharide and/or a monosaccharide;
- converting the cellulose in the lignocellulosic biomass into the oligosaccharide and/or the monosaccharide, wherein the aqueous slurry is exposed to a temperature from 45° C. to 60° C. during the converting;
- adding a gas to the aqueous slurry during the converting step for a time period so that the aqueous slurry comprises dissolved oxygen in an amount that inhibits the production of lactic acid by Lactobacillus bacteria within the aqueous slurry, wherein the gas comprises oxygen;
- and
- after the time period, combining the monosaccharide with yeast so that the yeast converts the monosaccharide into a biochemical.
2 more elements
A method of treating lignocellulosic biomass, wherein the method comprises:
- providing an aqueous slurry comprising the lignocellulosic biomass and one or more enzymes, wherein the lignocellulosic biomass comprises cellulose, and wherein the one or more enzymes can convert the cellulose into an oligosaccharide and/or a monosaccharide;
- converting the cellulose in the lignocellulosic biomass into the oligosaccharide and/or the monosaccharide;
- adding a gas to the aqueous slurry during the converting step for a time period so that the aqueous slurry comprises dissolved oxygen in an amount that inhibits the production of lactic acid by Lactobacillus bacteria within the aqueous slurry, wherein the gas comprises oxygen, wherein the dissolved oxygen is present in the aqueous slurry in an amount of at least 11 milligrams of dissolved oxygen per liter of slurry;
- and
- after the time period, combining the monosaccharide with yeast so that the yeast converts the monosaccharide into a biochemical.
2 more elements
Dependencies are inferred from the claim text.