Bioproduction of phenethyl alcohol, aldehyde, acid, amine, and related compounds

A method for bioproduction of substituted or unsubstituted phenylacetaldehyde, 2-phenylethanol, phenylacetic acid or phenylethylamine by one or more recombinant bacterial or fungal cells genetically engineered to overexpress, relative to a wild type cell, at least one enzyme, which method comprises subjecting a starting material to a plurality of enzyme-catalyzed chemical transformations in an one-pot reaction system, wherein the starting material is selected from the group consisting of glucose, L-phenylalanine, substituted L-phenylalanine, styrene and substituted styrene, wherein the genetically engineered cells:

• i) overexpress styrene monooxygenase and styrene oxide isomerase for generating substituted or unsubstituted phenylacetaldehyde from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs:
• 1 and 2;
• and the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:
• 3;
• or
• ii) overexpress styrene monooxygenase, styrene oxide isomerase and an aldehyde dehydrogenase for generating substituted or unsubstituted phenylacetic acid from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs:
• 1 and 2;
• the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:
• 3;
• and the aldehyde dehydrogenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:
• 4;
• or
• iii) overexpress styrene monooxygenase, styrene oxide isomerase, an aldehyde reductase and/or an alcohol dehydrogenase for generating substituted or unsubstituted 2-phenylethanol from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs:
• 1 and 2;
• the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:
• 3;
• and the alcohol dehydrogenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:
• 5;
• or
• iv) overexpress styrene monooxygenase, styrene oxide isomerase and a transaminase for generating substituted or unsubstituted phenylethylamine from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs:
• 1 and 2;
• the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:
• 3;
• and the transaminase is ω-transaminase comprising an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO:
• 6.