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US 11,597,946 B2Granted
AAV chimeras
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsMavis Agbandje-McKenna, Mario Mietzsch, Robert McKenna
Life of this patent
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Abstract
Provided herein are compositions and methods for packaging a recombinant adeno-associated vims (rAAV) particle comprising using inverted terminal repeats (ITRs) and rep genes of different serotypes and/or using chimeric rep genes.
Claims5
A composition comprising:
- a nucleic acid sequence comprising a rep gene that encodes a chimeric rep protein, wherein the rep gene comprises at least one nucleic acid sequence from a rep gene of a first AAV serotype and at least one nucleic acid sequence from a rep gene of a second AAV serotype, wherein the first AAV serotype is AAV1 or AAV8, wherein the second AAV serotype is AAV2, and wherein the chimeric rep protein exhibits a higher rAAV nucleic acid packaging efficiency when introduced into a cell as compared to a nonchimeric rep protein of said first or second AAV serotype.
Dependencies are inferred from the claim text.