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US 11,597,935 B2Granted
Building designer RNA nano-structures for synthetic biology applications
AssigneeBaylor College of Medicine
InventorLaising Yen
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AssigneeBaylor College of Medicine
InventorLaising Yen
Estimating the term…
Embodiments of the disclosure include compositions and methods for generating RNA nanostructures, particularly in a cell. In particular embodiments, RNA subunits comprising at least one three-way junction and at least one kissing loop are configured such that multiple RNA subunits can polymerize into a specific structure. In particular embodiments, the RNA subunits are configured such that sequence of at least one kissing loop is complementary to sequence of another kissing loop, such as on another RNA subunit, and the summation of multiple RNA subunits having specific individual structures results in a combined polymerized structure of a defined shape. In specific embodiments, an RNA nanostructure generated from methods herein is utilized for an application, such as manufacturing or genetic modifications in a cell.
A plurality of RNA molecules comprising at least one three-way junction and at least one kissing loop, wherein at least two RNA molecules in the plurality are bound to each other through complementary binding of a kissing loop on each RNA molecule, wherein when the loops are kissing between separate RNA molecules in the plurality, the arms of the respective kissing loops from the separate RNA molecules are at a substantially 180 degree angle.
A structure comprising a plurality of RNA units configured in a pattern of one, two, or three dimensions, wherein the RNA units comprise one or more RNA molecules, wherein the one or more RNA molecules comprise at least one three-way junction and at least one kissing loop wherein when the loops are kissing between separate RNA molecules in the plurality, the arms of the respective kissing loops from the separate RNA molecules are at a substantially 180 degree angle.
Dependencies are inferred from the claim text.