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US 11,597,921 B2Granted
Automated cell processing methods, modules, instruments, and systems
AssigneeInscripta, Inc.
InventorsJorge Bernate, Kevin Ness, Phillip Belgrader, Don Masquelier, Ryan Gill
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Abstract
In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
Claims30
An automated stand-alone multi-module cell editing instrument comprising:
- a receptacle configured to receive cells;
- one or more receptacles configured to receive expression vectors, wherein the expression vectors introduce a desired DNA change to the target region and remove a proto-spacer motif (PAM) region from the target region;
- a growth module for growing the cells for transformation;
- a cell concentration module to concentrate the cells;
- a transformation module configured to introduce nucleic acids into the cells by bead transfection;
- a selection module;
- a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells using an RNA-guided nuclease;
- a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script;
- and
- an automated liquid handling system to move liquids from the receptacle to the growth module, from the growth module to the cell concentration module, from the cell concentration module to the transformation module, from the transformation module to the selection module, and from the selection module to the nuclease-directed editing module, without user intervention.
7 more elements
An automated stand-alone multi-module cell editing instrument for RNA-guided editing of mammalian cells comprising:
- a receptacle configured to receive mammalian cells;
- one or more receptacles configured to receive expression vectors, wherein the expression vectors introduce a desired DNA change to the target region and remove a proto-spacer motif (PAM) region from the target region;
- a growth module for growing the mammalian cells for transformation;
- a cell concentration module to concentrate the mammalian cells;
- a transformation module configured to introduce nucleic acids into the mammalian cells by bead transfection;
- a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells using an RNA-guided nuclease;
- a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script;
- and
- an automated liquid handling system to move liquids from the receptacle to the growth module, from the growth module to the cell concentration module, from the cell concentration module to the transformation module, and from the transformation module to the nuclease-directed editing module, without user intervention.
6 more elements
Dependencies are inferred from the claim text.