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AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College, The University of Tokyo
InventorsSilvana Konermann, Alexandro Trevino, Mark Brigham, Fei Ran, Patrick Hsu, Chie-yu Lin, Osamu Nureki, Hiroshi Nishimasu, Ryuichiro Ishitani, Feng Zhang
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The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.
A CRISPR-Cas system RNA comprising a guide sequence capable of hybridizing to a target sequence in a genomic locus of interest in a cell, and a modified loop comprising an insertion of a distinct RNA sequence capable of binding to an adaptor protein into a tetraloop and/or a stem-loop 2 of the CRISPR-Cas system RNA.
Dependencies are inferred from the claim text.