Nicotine degrading enzyme variants
AssigneesAntidote Therapeutics, Inc., Board of Regents, The University of Texas System
InventorsMatthew W. Kalnik, Thomas Thisted, Everett Stone, Charles C. Reed, Max Rodnick-Smith
Life of this patent
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Abstract
Described are nicotine-degrading enzyme variants that exhibit increased nicotine-degrading activity and/or decreased immunogenicity relative to the wild-type NicA2 and NOX enzymes, compositions comprising the variants, and methods using them.
Claims24
A nicotine-degrading enzyme variant comprising an amino acid sequence that is a variant of the amino acid sequence of the wild-type NicA2 enzyme set forth in SEQ ID NO:
- 1, wherein the variant sequence has at least 90% sequence identity to a sequence selected from (i) SEQ ID NO:
- 1 and (ii) SEQ ID NO:
- 1 having an N-terminal deletion of up to 52 amino acids, and wherein the variant comprises at least one substitution at an amino acid position selected from positions 91, 104, 106, 107, 217, 250, 340, 366, 381, 427, 462, and 463 of SEQ ID NO:1, and has nicotine-degrading activity.
A nicotine-degrading enzyme variant having nicotine-degrading activity and comprising an amino acid sequence having at least about 95% sequence identity to a sequence selected from (i) SEQ ID NO:
- 1 and (ii) SEQ ID NO:
- 1 having an N-terminal deletion of up to 52 amino acids, wherein said variant comprises at least one substitution at an amino acid position selected from the group consisting of positions 91, 104, 106, 107, 217, 250, 340, 366, 381, 427, 462, and 463 of SEQ ID NO:1.
A nicotine-degrading enzyme variant having nicotine-degrading activity and comprising an amino acid sequence of:
- (i) SEQ ID NO:
- 1 with at least one substitution at one or more amino acid positions selected from the group consisting of positions 91, 104, 106, 107, 217, 250, 340, 366, 381, 427, 462, and 463 of SEQ ID NO:1, or
- (ii) SEQ ID NO:
- 1 having an N-terminal deletion of up to 52 amino acids and at least one substitution at one or more amino acid positions selected from the group consisting of positions 91, 104, 106, 107, 217, 250, 340, 366, 381, 427, 462, and 463 of SEQ ID NO:1.
Dependencies are inferred from the claim text.