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US 11,597,913 B2Granted
Methods for generating hematopoietic stem cells
AssigneeThe Brigham and Women's Hospital, Inc.
InventorDhvanit I. Shah
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Abstract
In some aspects and embodiments, the invention provides methods for making hematopoietic stem cells, including for HSCT. The method comprises providing a cell population comprising hemogenic endothelial (HE) or endothelial cells, and increasing activity or expression of DNA (cytosine-5-)-methyltransferase 3 beta (Dnmt3b) and/or GTPase IMAP Family Member 6 (Gimap6) in the HE and/or endothelial cells under conditions sufficient for stimulating formation of HSCs.
Claims16
A method of preparing hematopoietic stem cells (HSCs), the method comprising:
- providing a cell population comprising hemogenic endothelial (HE) cells, and increasing the expression of DNA (cytosine-5-)methyltransferase 3 beta (Dnmt3b) in the cell population ex vivo under conditions sufficient for stimulating formation of HSCs, wherein the cell population is not subjected to circumferential stretching.
Dependencies are inferred from the claim text.