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US 11,597,906 B2Granted
System for obtaining protein-rich nutrient supplements from bacterial fermentation process
AssigneeJupeng Bio (HK) Limited
InventorsRyan Senaratne, McKinzie Fruchtl, Abel Price
Life of this patent
Estimating the term…
Abstract
Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.
Claims20
A system for producing a protein-rich nutrient supplement from a fermentation process using anaerobic bacteria, comprising:
- a fermentation vessel connected to a gas inlet line for flowing a gaseous substrate into the fermentation vessel and a liquid inlet line for supplying a culture medium into the fermentation vessel containing the anaerobic bacteria to ferment the gaseous substrate and the culture medium into a fermentation liquid broth;
- a first cell separator connected to one or more first outlet lines of the fermentation vessel to receive a first flow of the fermentation liquid broth from the fermentation vessel and separate the first flow of the fermentation liquid broth into a first cell-containing suspension and a first cell-free permeate solution;
- a processing chamber connected to one or more second outlet lines of the first cell separator to receive the first cell-free permeate solution from the first cell separator and process the first cell-free permeate solution into an oxygenated hydrocarbonaceous compound, wherein the processing chamber is a distillation chamber;
- a second cell separator connected to one or more second outlet lines of the fermentation vessel to receive a second flow of the fermentation liquid broth from the fermentation vessel and separate the second flow of the fermentation liquid broth into a second cell-containing suspension and second cell-free permeate solution;
- one or more cell rupturing devices connected to the second cell separator to receive the second cell-containing suspension, and rupture cell membranes of the second cell-containing suspension to generate a homogenate, wherein the rupturing of the cell membranes is accomplished by one or more rupturing devices selected from the group consisting of a microfluidic device, a sonication device, an ultrasonic device, a French press, and combinations thereof;
- and
- one or more fractionators connected to one or more third outlet lines of the one or more cell rupturing devices to receive the homogenate from the one or more cell rupturing devices and fractionate the homogenate into a first protein-containing portion and a protein-containing cell debris portion.
4 more elements
A system for producing a protein-rich nutrient supplement from a fermentation process using anaerobic bacterial, comprising:
- a fermentation vessel connected to a gas line for flowing a gaseous substrate into the fermentation vessel and a liquid inlet line for supplying a culture medium into the fermentation vessel containing the anaerobic bacterial to ferment the gaseous substrate and the culture medium into a fermentation liquid broth;
- a first cell separator connected to one or more first outlet lines of the fermentation vessel to receive a first flow of the fermentation liquid broth and separate the first flow of the fermentation liquid broth into a first cell-containing suspension and a first cell-free permeate solution;
- a cell-free holding tank connected to one or more second outlet lines of the first cell separator to receive the first cell-free permeate solution;
- a processing chamber connected to one or more third outlet lines of the cell-free holding tank to receive the first cell-free permeate solution from the cell-free holding tank and process the first cell-free permeate solution into an oxygenated hydrocarbonaceous compound, wherein the processing chamber is a distillation chamber;
- a second cell separator connected to one or more second outlet lines of the fermentation vessel to receive a second flow of the fermentation liquid broth from the fermentation vessel and separate the second flow of the fermentation liquid broth into a second cell-containing suspension and second cell-free permeate solution;
- one or more cell rupturing devices to receive the second cell-containing suspension, rupture cell membranes of cells contained within the second cell-containing suspension, and generate a homogenate, wherein the rupturing of the cell membranes is accomplished by one or more rupturing devices selected from the group consisting of a microfluidic device, a sonication device, an ultrasonic device, a French press, and combinations thereof;
- and
- one or more fractionators connected to one or more fourth outlet lines of the one or more cell rupturing devices to receive the homogenate from the one or more cell rupturing devices and fractionate the homogenate into a first protein-containing portion and a protein-containing cell debris portion.
5 more elements
A system for producing a protein-rich nutrient supplement from a fermentation process using anaerobic bacterial, comprising:
- a fermentation vessel connected to a gas inlet line for flowing a gaseous substrate into the fermentation vessel and a liquid inlet line for supplying a culture medium into the fermentation vessel to ferment the gaseous substrate and the culture medium into a fermentation liquid broth;
- a first cell separator connected to one or more first outlet lines of the fermentation vessel to receive a first flow of the fermentation liquid broth from the fermentation vessel and separate the first flow of the fermentation liquid broth into a first cell-containing suspension and a first cell-free permeate solution;
- a processing chamber connected to one or more second outlet lines of the first cell separator to receive the first cell-free permeate solution from the first cell separator and process the first cell-free permeate solution into an oxygenated hydrocarbonaceous compound, wherein the processing chamber is a distillation chamber;
- a second cell separator connected to the fermentation vessel to receive a second flow of a second fermentation liquid from the fermentation vessel and separate the second flow of the second fermentation liquid into a second cell-containing suspension and a second cell-free permeate solution;
- a cell-containing holding tank connected to the second cell separator to receive the second cell-containing suspension;
- one or more cell rupturing devices to receive the second cell-containing suspension and generate a homogenate of the second cell-containing suspension, wherein the rupturing of the cell membranes is accomplished by one or more rupturing devices selected from the group consisting of a microfluidic device, a sonication device, an ultrasonic device, a French press, and combinations thereof;
- and
- one or more fractionators connected to the one or more cell rupturing devices to receive the homogenate and separate the homogenate into a first protein-containing portion and a protein-containing cell debris portion.
5 more elements
Dependencies are inferred from the claim text.