Liquid filtration system with integrated bleed function
AssigneeThe Automation Partnership (Cambridge) Limited
InventorPaul Grant
Life of this patent
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Abstract
A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required.
Claims12
A liquid filtration system for perfusion of a cell culture comprising:
- a bioreactor for holding a cell culture liquid;
- a filter configured to filter cell culture liquid passing therethrough;
- a perfusion pump configured to move cell culture liquid between the bioreactor and the filter;
- a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter;
- and
- a bleed outlet provided on the liquid line as an additional outlet in the perfusion pump, the bleed outlet configured to provide means to remove cell culture liquid selectively from the system under the action of the perfusion pump.
A method for removing cell culture liquid from a liquid filtration system, the system comprising:
- a bioreactor for holding cell culture liquid;
- a filter configured to filter cell culture liquid passing therethrough;
- a perfusion pump configured to move cell culture liquid between the bioreactor and the filter;
- a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter;
- and
- a bleed outlet provided on the liquid line as an additional outlet in the perfusion pump, the bleed outlet configured to provide means to remove cell culture liquid selectively from the system;
- the method comprising:
- operating the perfusion pump such that cell culture liquid is moved through the filter via the liquid line;
- opening the bleed outlet such that the cell culture liquid is removed from the liquid line under the action of the perfusion pump;
- closing the bleed outlet after a desired amount of cell culture liquid has been removed from the system.
Dependencies are inferred from the claim text.