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US 11,597,901 B2Granted
Adapter for cell-culture vessel
AssigneesBayer Aktiengesellschaft, Bayer HealthCare LLC
InventorsAnnette Eidt, Wolf Kloeckner, Helmut Brod, Martin Poggel, Bernd Tscheschke
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Abstract
Disclosed herein is a sterilizable adapter for a cell culture vessel comprising a body, an upper connection and/or a lid, at least one port, a ventilation passage, a lower internal connection suitable for connection to a cell culture vessel and a cell retention device and/or a cell separation device as well as a device comprising said sterilizable adapter and a method for cell cultivation using said sterilizable adapter.
Claims9
A sterilizable adapter for a small-scale cell culture vessel comprising:
- a body, an upper connection and/or a lid,
- at least one port for medium addition,
- at least one second port for medium removal,
- a ventilation passage,
- a lower internal connection suitable for connection to the cell culture vessel and
- at least one cell retention device,
- wherein the cell culture vessel is a shake flask configured to removably receive the sterilizable adapter and the sterilizable adapter is a sterilizable adapter for a small-scale cell culture, with a cultivation volume of 1 ml to 500 ml,
- wherein the at least one cell retention device is a gravity cell settler and is inclined with an angle between 60° and 80° with respect to a horizontal line;
- and wherein the shake flask and adapter in use may be moved by constant orbital rotational movement at a rate of from 100 to 500 revolutions per minute.
6 more elements
Dependencies are inferred from the claim text.