Gas mixer and pressure apparatus

A system of delivering a gas mixture to at least one microfluidic device, comprising:

• 1) an apparatus comprising i) a gas mixer configured to mix gas from at least two gas sources into a gas mixture, ii) at least one pneumatic pressure generator configured to generate at least one pneumatic pressure and iii) conduits configured to deliver said gas mixture and said at least one pneumatic pressure to 2) a culture module comprising a moveable pressure manifold and 3) at least one microfluidic device;
• wherein said at least one pneumatic pressures is configured to actuate a movement of said moveable pressure manifold in said culture module, and wherein said gas mixture is configured to provide culture conditions in said at least one microfluidic device.

A system of delivering a gas mixture to at least one microfluidic device, comprising:

• 1) an apparatus comprising i) a gas mixer configured to mix gas from at least two gas sources into a gas mixture, ii) at least one pneumatic pressure generator configured to generate at least one pneumatic pressure and iii) conduits configured to deliver said gas mixture and said at least one pneumatic pressure to 2) a culture module and 3) at least one microfluidic device comprising cells on a membrane;
• wherein said at least one pneumatic pressures is configured to actuate a movement of said membrane in said at least one microfluidic device, and wherein said gas mixture is configured to provide culture conditions in said at least one microfluidic device.