US 11,597,898 B2Granted

Number analyzing method, number analyzing device, and storage medium for number analysis

AssigneeAipore Inc.

InventorsTakashi Washio, Tomoji Kawai, Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Akira Ishii, Takeshi Yoshida

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Abstract

A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

Claims6

Dependent claims

A number analyzing method comprising:

arranging a partition wall with a through-hole and electrodes disposed on a front side and a back side of the partition wall through the through-hole;
supplying a flowable material containing particulate or molecular analytes to one side of the partition wall;
obtaining detection signals of an electrical conduction change between the electrodes, the electrical conduction charge caused by passage of the particulate or molecular analytes through the through-hole;
and
analyzing a number of each analyte type of the particulate or molecular analytes by executing a computer control program based on data of the detection signals, and
wherein the computer control program is configured to perform a probability density estimation from a data group based on feature values indicating feature of waveforms of pulse signals obtained as the detection signals which correspond to the passage of the particulate or molecular analytes,
wherein the computer control program includes a number deriving means configured to derive the number of each analyte type,
wherein a feature value of the feature values is a first type showing local feature of waveforms of pulse signals, and
wherein the feature value of the first type is:
a wave height value of a waveform in a predetermined time width,
a pulse wavelength ta,
a peak position ratio represented by ratio tb/ta of time ta and tb leading from the pulse start to a pulse peak,
a kurtosis which represents a sharpness of the waveform,
a depression representing a slope leading from a pulse start to a pulse peak,
an area representing total sum of a time division area dividing the waveform with predetermined times, or
an area ratio of sum of the time division area leading from the pulse start to the pulse peak to the total waveform area.

13 more elements

A number analyzing device comprising:

a partition wall with a through-hole;
electrodes disposed on a front side and a back side of the partition wall through the through-hole;
a flowable material containing particulate or molecular analytes supplied to one side of the partition wall;
a computer control program configured to analyze the number of each analyte type based on a data of detection signals when the detection signals are obtained through an electrical conduction change caused between the electrodes by passage of the particulate or molecular analytes through the through-hole;
a storage means configured to control the data of detection signals;
and
a control means configured to control execution of the computer control program based on the data of detection signals;
and
wherein the computer control program is configured to perform a probability density estimation from a data group based on feature values indicating feature of waveforms of pulse signals obtained as the detection signals which correspond to the passage of the particulate or molecular analytes,
wherein the computer control program includes a number deriving means configured to derive the number of each analyte type,
wherein a feature value of the feature values is a first type showing local feature of waveforms of pulse signals, and
wherein the feature value of the first type is:
a wave height value of a waveform in a predetermined time width,
a pulse wavelength ta,
a peak position ratio represented by ratio tb/ta of time ta and tb leading from the pulse start to a pulse peak,
a kurtosis which represents a sharpness of the waveform,
a depression representing a slope leading from a pulse start to a pulse peak,
an area representing total sum of a time division area dividing the waveform with predetermined times, or
an area ratio of sum of the time division area leading from the pulse start to the pulse peak to the total waveform area.

16 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/065,633Filed 19 Dec 2016
Priority: JP JP2015-25439825 Dec 2015
Published: US 2019/0257787 A122 Aug 2019
Granted: 7 Mar 2023
Term ends: Not estimatedThe source records don’t support an estimate.

Classification17 codes

C12M 1/34
Apparatus for enzymology or microbiology: measuring or testing with condition measuring or sensing means, e.g. colony counters
C12Q 1/04
Involving viable microorganisms: determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides;…
G01N 15/12
By measuring electrical or magnetic effects: by observing changes in resistance or impedance across apertures when traversed by individual…
G01N 33/48721
By electrical means: investigating individual macromolecules, e.g. by translocation through nanopores