Anti-E-selectin antibodies, compositions and methods of use
AssigneePfizer Inc.
InventorsJames Reasoner Apgar, Sheryl Rubio Bowley, Joanne Elizabeth-Ayriss Elwell, Laura Lin, Jatin Narula, Chuenlei Parng, Debra Denene Pittman, Swapnil Rakhe, Chihyi Vincent Yu
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Abstract
The invention provides antibodies, and antigen-binding fragments thereof, that specifically bind to E-selectin. The invention includes uses, and associated methods of using the antibodies, and antigen-binding fragments thereof.
Claims24
An isolated antibody that specifically binds to human E-selectin, comprising at least one of the following:
- (a) a light chain complementarity determining region 1 (LCDR-1) comprising the amino acid sequence of SEQ ID NO:2, a LCDR-2 comprising the amino acid sequence of SEQ ID NO:3, a LCDR-3 comprising the amino acid sequence of SEQ ID NO:4, a heavy chain complementarity determining region 1 (HCDR-1) comprising the amino acid sequence of SEQ ID NO:8, a HCDR-2 comprising the amino acid sequence of SEQ ID NO:9, and a HCDR-3 comprising the amino acid sequence of SEQ ID NO:10;
- (b) a heavy chain variable domain (VH) comprising the amino acid sequence of SEQ ID NO:11 and a light chain variable domain (VL) comprising the amino acid sequence of SEQ ID NO:5;
- and
- (c) a heavy chain (HC) comprising the amino acid sequence of SEQ ID NO:7 or SEQ ID NO:13, and a light chain (LC) comprising the amino acid sequence of SEQ ID NO:1.
An isolated antibody that specifically binds to human E-selectin, comprising:
- a VL amino acid sequence comprising a LCDR-1, a LCDR-2 and a LCDR-3 of the amino acid sequence of SEQ ID NO:5;
- and
- a VH amino acid sequence comprising a HCDR-1, a HCDR-2 and a HCDR-3 of the amino acid sequence of SEQ ID NO:11.
An isolated antibody that specifically binds to human E-selectin, comprising a LC comprising the amino acid sequence of SEQ ID NO:1, and a HC comprising the amino acid sequence of SEQ ID NO:7 or SEQ ID NO:13.
An isolated nucleic acid molecule encoding an antibody that specifically binds human E-selectin comprising the amino acid sequence of SEQ ID NO:5 and SEQ ID NO:11.
An isolated nucleic acid molecule comprising at least one of the following:
- a) the nucleic acid sequence of SEQ ID NO:136 and the nucleic acid sequence of SEQ ID NO:137;
- and
- b) a nucleic acid sequence of SEQ ID NO:138 or SEQ ID NO:206, and the nucleic acid sequence of SEQ ID NO:139.
A vector comprising a nucleic acid molecule comprising (a) the nucleic acid sequence of SEQ ID NO:136 and the nucleic acid sequence of SEQ ID NO:137 or (b) the nucleic acid sequence of SEQ ID NO:138 or SEQ ID NO:206, and the nucleic acid sequence of SEQ ID NO:139.
A host cell comprising a vector comprising a nucleic acid molecule comprising (a) the nucleic acid sequence of SEQ ID NO:136 and the nucleic acid sequence of SEQ ID NO:137 or (b) the nucleic acid sequence of SEQ ID NO:138 or SEQ ID NO:206, and the nucleic acid sequence of SEQ ID NO:139.
A method of making an antibody comprising culturing a host cell under a condition wherein an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO:11 and a VL comprising the amino acid sequence of SEQ ID NO:5 is produced by the host cell.
Dependencies are inferred from the claim text.