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US 11,596,908 B2Granted
Droplet libraries
AssigneeBio-Rad Laboratories, Inc.
InventorsDarren Roy Link, John Brian Hutchison, Michael L. Samuels, Michael Weiner
Life of this patent
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Abstract
The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.
Claims16
A method for detecting target molecules in a fluid sample, the method comprising:
- partitioning, in a microfluidic device, a fluid sample comprising target molecules into partitions, wherein a plurality of the partitions each comprise:
- a target molecule;
- a bead comprising a first antibody capable of binding the target molecule and containing a unique label;
- and
- a second antibody linked to a readout enzyme and capable of binding to the target molecule bound to the bead thereby to form an immunocomplex, wherein each of the plurality of partitions includes exactly one immunocomplex;
- introducing a fluorogenic substrate to the immunocomplex in each partition;
- and
- simultaneously detecting in each partition a fluorescent signal produced in said introducing step, and an optical signal from the unique label.
5 more elements
A method for detecting a protein, the method comprising:
- introducing a test fluid into partitions from an antibody library, wherein each partition comprises an optically-labeled, substrate-bound first antibody against a target antigen and an enzyme-linked second antibody against the target antigen;
- forming at least one immunocomplex with the first and the second antibodies;
- recovering beads from the partitions;
- washing the beads;
- isolating individual beads into ELISA assay droplets;
- converting, by an enzyme of the enzyme-linked second antibody, fluorogenic substrate into a fluorescent signal in one droplet of the ELISA assay droplets;
- and
- simultaneously reading from the one droplet the fluorescent signal and an optical signal from the optically-labeled, substrate-bound first antibody, wherein the optical signal uniquely identifies the first antibody.
5 more elements
Dependencies are inferred from the claim text.