Irradiated membrane for cell expansion
AssigneeGambro Lundia AB
InventorsBernd Krause, Markus Neubauer, Joachim Loercher
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Abstract
The invention relates to a membrane which can be used for cultivating adherent or suspension cells, in particular adherent cells, wherein said membrane allows for the adhesion and proliferation of the cells due to the irradiation of the wet or dry membrane with gamma- or beta-rays or an electron beam in a dose of from 12.5 to 175 kGy in the presence of oxygen. The resulting membrane may be used without any pre-treatment with surface-modifying substances. The invention further relates to a method for preparing said irradiated membrane which can be used for the cultivation of cells, in particular adherent cells, and to methods of using such a membrane for the cultivation of cells, in particular adherent cells.
Claims20
A method for the cultivation of adherent cells, the method comprising the steps of:
- preparing a membrane comprising i) a component selected from the group consisting of a polysulfone, polyethersulfone, a polyarylethersulfone, and combinations thereof, and ii) a polyvinylpyrrolidone,
- irradiating the prepared membrane with at least one of gamma-rays, beta-rays, and an electron beam in a dose of from 12.5 to 175 kGy in the presence of oxygen at a concentration of 4 to 100 vol %, and
- inoculating the irradiated membrane with the adherent cells to be cultivated.
A method for the extracorporeal treatment of body fluids, the method comprising the steps of:
- preparing a membrane comprising i) at least one of a polysulfone, a polyethersulfone, and a polyarylethersulfone, and ii) a polyvinylpyrrolidone,
- irradiating the prepared membrane with at least one of gamma-rays, beta-rays, and an electron beam in a dose of from 12.5 to 175 kGy in the presence of oxygen at a concentration of 4 to 100 vol %, and
- inoculating the irradiated membrane with adherent cells for treating the body fluid.
Dependencies are inferred from the claim text.