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US 11,596,714 B2Granted
Methods for development and use of minimally polarized function cell micro-aggregate units in tissue applications using LGR4, LGR5 and LGR6 expressing epithelial stem cells
AssigneePolarityTE, Inc.
InventorDenver M. Lough
Life of this patent
Estimating the term…
Abstract
Provided herein are constructs of micro-aggregate multicellular, minimally polarized grafts containing Leucine-rich repeat-containing G-protein coupled Receptor (LGR) expressing cells for wound therapy applications, tissue engineering, cell therapy applications, regenerative medicine applications, medical/therapeutic applications, tissue healing applications, immune therapy applications, and tissue transplant therapy applications which preferably are associated with a delivery vector/substrate/support/scaffold for direct application.
Claims20
A tissue regenerative composition, comprising:
- a first tissue section comprising a dermal segment, an epidermal segment, and a segment of a follicular unit of mammalian cutaneous tissue specimen, wherein the dermal segment and the epidermal segment are outside the follicular unit;
- the segments are interconnected;
- and
- a second tissue section separated from the first tissue section, the second tissue section consisting of a second dermal segment and a second epidermal segment, wherein the second dermal segment and the second epidermal segment are interconnected;
- wherein the segment of the follicular unit comprises living LGR-expressing stem cells that are exposed to the second tissue section.
2 more elements
A tissue regenerative composition, comprising:
- a tissue section comprising a dermal segment, an epidermal segment, and a segment of a follicular compartment of mammalian cutaneous tissue specimen, wherein the segments are interconnected and the segment of the follicular compartment comprises living LGR-expressing stem cells that are exposed;
- wherein the composition is prepared by a process comprising separating fat and hypodermal elements from the mammalian cutaneous tissue specimen ex vivo to provide remaining cutaneous elements containing an epidermal compartment, a dermal compartment, and the follicular compartment;
- and segmenting the epidermal compartment, the dermal compartment, and the follicular compartment to open the follicular compartment and prepare the tissue section.
Dependencies are inferred from the claim text.