Solubilization of antigen components for removal from tissues
AssigneeThe Regents of the University of California
InventorsLeigh G. Griffiths, Angeliki Papalamprou, Maelene L. Wong
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for removing antigens from tissues by sequentially destabilizing and/or depolymerizing cytoskeletal components and removing and/or reducing water-soluble antigens and lipid-soluble antigens. The invention further relates to tissue scaffolding and decellularized extracellular matrix produced by such methods.
Claims20
A repopulated decellularized extracellular matrix (ECM) scaffold depleted of antigens comprising:
- (a) a decellularized ECM scaffold depleted of antigens comprising an ECM structure and an ECM tensile strength that is substantially the same as the ECM prior to antigen removal, wherein the ECM is substantially free of endogenous antigens, wherein a least 60% of lipid-soluble antigens are removed from the ECM, and wherein the glycosaminoglycan (GAG) content is substantially decreased in comparison to the ECM prior to antigen removal, and
- (b) live exogenous cells,
- wherein the decellularized ECM scaffold is repopulated with the live exogenous cells.
A repopulated decellularized extracellular matrix (ECM) scaffold comprising a decellularized ECM scaffold depleted of antigens and repopulated with live exogenous cells,
- wherein the ECM is substantially free of endogenous antigens, and
- wherein the decellularized ECM scaffold comprises:
- i) ECM tensile strength, ECM collagen and ECM elastin that is substantially the same as the ECM prior to antigen removal;
- ii) substantially reduced glycosaminoglycan (GAG) content in comparison to a native ECM prior to antigen removal;
- and
- iii) at least 60% reduction of lipid-soluble antigens in comparison to a native tissue control.
Dependencies are inferred from the claim text.