Compositions and methods for organ-protective expression and modulation of coding ribonucleic acids
AssigneeCombined Therapeutics, Inc.
InventorsRomain Micol, Valerie Duval
Life of this patent
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Abstract
Compositions are provided comprising messenger RNA constructs having at least one open reading frame (ORF), wherein the ORF is operatively linked to at least one untranslated region (UTR), wherein the UTR comprises at least one organ protection sequence (OPS), wherein the OPS sequence comprises at least a first, a second and a third micro-RNA (miRNA) target sequence, and wherein each of the at least a first, second and third the miRNA target sequences are optimised to hybridise with a corresponding miRNA sequence. The compositions and molecules provided are useful in therapies such as for the treatment of cancer, in immunotherapies, and in vaccines.
Claims18
A composition comprising:
- at least a first mRNA construct comprising at least a first open reading frame (ORF), wherein the ORF is operatively linked to at least one untranslated region (UTR), wherein the UTR comprises at least a first, second, and a third micro-RNA (miRNA) target sequence, and wherein each of the at least first, second, and third miRNA target sequences are optimised to each hybridise with a different corresponding miRNA sequence;
- further comprising at least a second mRNA construct comprising at least a second open reading frame (ORF), wherein the second ORF encodes a proinflammatory cytokine selected from the group consisting of:
- IL-1;
- IL-2;
- IL-3;
- IL-4;
- IL-5;
- IL-6;
- IL-7;
- IL-8;
- IL-9;
- IL-10;
- IL-11;
- IL-12;
- IL-15;
- IL-17;
- IL-21;
- TGF-beta;
- IFNγ;
- IFNα;
- IFNβ;
- TNFα;
- M-CSF;
- G-CSF;
- and GM-CSF,
- wherein the first ORF encodes an antigen selected from:
- a bacterial protein and/or a viral protein;
- and
- an in vivo delivery composition;
- wherein the first and second mRNA constructs are comprised within or adsorbed to the delivery composition.
Dependencies are inferred from the claim text.