Removal of agglomerates
AssigneeSeqirus Pty Ltd
InventorsSteven Rockman, Jesse Bodle, Nancy Guzzo-Pernell
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Abstract
The present disclosure provides a method of dispersing agglomerated material in a preparation comprising influenza proteins. The method comprises subjecting the preparation to sonication.
Claims20
A method of dispersing agglomerated material in a preparation comprising influenza virus proteins, the method comprising subjecting the preparation to sonication;
- wherein the sonication is conducted at a rate of 80% amplitude.
A method of producing an influenza virus vaccine, the method comprising producing a preparation comprising inactivated or split influenza virions and sonicating the preparation;
- wherein the sonication is conducted at a rate of 80% amplitude.
A method of dispersing agglomerated material in a preparation comprising influenza virus proteins, the method comprising subjecting the preparation to sonication;
- wherein the sonication is conducted to transfer at least 90 Joules/mL of energy.
A method of dispersing agglomerated material in a preparation comprising influenza virus proteins, the method comprising subjecting the preparation to sonication;
- wherein influenza virus H3N2 strains that have a predicted HA glycosylation site probability score (pGly score) ≥16 require sonication of ≥90 Joules/mL and H1N1 strains that have a pGly score ≥11 require sonication of ≥90 Joules/mL.
Dependencies are inferred from the claim text.