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US 11,596,677 B2Granted
Induction of protective immunity against antigens
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsRoy Curtiss, III, Shifeng Wang
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Abstract
Described herein are compositions and methods for making and using recombinant bacteria that are capable of regulated attenuation and/or regulated expression of one or more antigens from Clostridium Perfringens as vaccines to prevent necrotic enteritis (NE).
Claims17
A recombinant Salmonella enterica subsp. Enterica bacterium comprising a nucleic acid comprising:
- a sequence encoding a choloylglycine hydrolase (Cbh) antigen, or antigenic fragment thereof, and/or a sequence encoding a Clostridium perfringens enterotoxin (CpeC) antigen, or antigenic fragment thereof, wherein the recombinant bacteria further comprises:
- an araC ParaBAD-regulated murA gene;
- a deletion-insertion mutation that inactivates the expression of asdA gene and inserts a c2 gene;
- a deletion in a pmi gene;
- a deletion-insertion mutation that inactivates the expression of a RelA gene and inserts a lacI gene;
- and
- a deletion in a sifA gene.
4 more elements
Dependencies are inferred from the claim text.