Delivery of RNA to trigger multiple immune pathways

A composition comprising lipid particles and messenger ribonucleic acid (mRNA) molecules;

• the mRNA molecules comprising a 5′ cap nucleoside, a first 5′ ribonucleoside, a triphosphate bridge, and a sequence that encodes an immunogen;
• the immunogen comprising a respiratory syncytial virus (RSV) surface fusion glycoprotein (F-protein) immunogen, an Epstein-Barr virus glycoprotein immunogen, a cytomegalovirus glycoprotein immunogen, a coronavirus spike polypeptide immunogen, an influenza virus immunogen, a Varicella zoster virus glycoprotein immunogen, a human papillomavirus 16 (HPV16) E6 immunogen, a HPV 16 E7 immunogen, or a flavivirus immunogen;
• the first 5′ ribonucleoside comprising a 2′-methylated ribose;
• the 5′ cap nucleoside being linked 5′-to-5′ to the first 5′ ribonucleoside by the triphosphate bridge;
• the lipid particles comprising:
• (a) a polyethylene glycol-ylated (PEGylated) lipid, (b) cholesterol, (c) an anionic phospholipid or a zwitterionic phospholipid, and (d) a cationic lipid comprising a tertiary amine;
• and the lipid particles encapsulating at least half of the mRNA molecules.