Anti-freezing agent for protecting biological tissue during freezing treatment and preparation method therefor
AssigneeTaipei Veterans General Hospital
InventorsPo Kuei Wu, Wei Ming Chen, Cheng Fong Chen, Jir You Wang, Wen Hai Wu
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Abstract
Disclosed are an anti-freezing agent for protecting a biological tissue from being damaged during a freezing treatment and a preparation method therefor. The method for preparing the anti-freezing agent involves mixing ethylene glycol, water and dimethylsulfoxide homogeneously to form a matrix, and then slowly adding sodium polyacrylate. The prepared anti-freezing agent is coated onto biological tissue during a freezing treatment such that damage to the biological tissue is reduced.
Claims3
A method for protecting a biological tissue of a human or animal during a freezing treatment, comprising
- applying an anti-freezing agent to the biological tissue to protect the biological tissue from being damaged during the freezing treatment;
- wherein the anti-freezing agent is prepared by the following steps:
- (1) mixing ethylene glycol and water to form an aqueous ethylene glycol solution, and then adding dimethyl sulfoxide into the aqueous ethylene glycol solution and mixing uniformly to form a matrix, wherein a weight ratio of ethylene glycol to water is about 1:1 to 3:1, and a weight ratio of dimethyl sulfoxide to the aqueous ethylene glycol solution is about 1:10 to 2:10;
- and
- (2) heating the matrix slowly and then adding sodium polyacrylate into the heated matrix, which is then stirred uniformly and cooled to room temperature to obtain the anti-freezing agent, wherein a weight ratio of the matrix to sodium polyacrylate is 4:1 to 10:1.
Dependencies are inferred from the claim text.