Methods for biological sample processing and analysis
AssigneeUltima Genomics, Inc.
InventorsGilad Almogy, Kristopher Barbee, Nathan Beckett
Life of this patent
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Abstract
Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.
Claims29
A method for processing a biological analyte, comprising:
- (a) providing a substrate comprising said biological analyte immobilized thereto;
- (b) subsequent to (a), directing a solution comprising a plurality of probes from a dispensing unit to a fluid medium and from said fluid medium to a first location and a second location of said substrate, wherein said plurality of probes comprises a first probe with a detectable label and a second probe with no detectable label, and wherein said first location and said second location are disposed at different radial distances from a central axis of said substrate;
- (c) subsequent to (b), coupling said first probe of said plurality of probes to said biological analyte;
- and
- (d) detecting one or more signals or signal changes from said detectable label of said first probe coupled to said biological analyte to determine a sequence of said biological analyte.
Dependencies are inferred from the claim text.