Modified bacteria for production of nitroaromatics
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsYousong Ding, Ran Zuo
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Abstract
The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.
Claims11
A recombinant bacterial cell comprising one or more isolated nucleic acids engineered to express:
- (i) a fusion protein comprising a TxtE enzyme linked to a catalytic domain of a CYP102A1 (P450BM3) reductase enzyme via an amino acid linker sequence that is between 14 and 27 amino acids in length, wherein the fusion protein is a TB14 fusion protein having the sequence set forth in SEQ ID NO:
- 1;
- (ii) a nitric oxide synthase (NOS) enzyme;
- and, optionally
- (iii) a glucose dehydrogenase (GDH) enzyme.
An isolated nucleic acid comprising the sequence set forth in any one of SEQ ID NOs:
- 8-13.
Dependencies are inferred from the claim text.