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US 11,591,572 B2Granted
Differentiation method
AssigneeKoninklijke Nederlandse Akademie van Wetenschappen
InventorsJohannes Carolus Clevers, Helmuth Gehart
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Abstract
The invention relates to differentiation methods for progenitor cells, e.g. mammalian epithelial stem cells, differentiation media for use in said methods, organoids and cells obtainable by said methods and uses, including therapeutic uses, thereof.
Claims20
A method for differentiating liver progenitor cells, wherein said method comprises:
- culturing the cells in a differentiation medium comprising a basal medium, one or more receptor tyrosine kinase ligands, a Notch inhibitor, a glucocorticoid, a TGF-beta inhibitor, one or more Wnt inhibitors, and a Wnt agonist, wherein the Wnt agonist is a GSK-3 inhibitor, and wherein the one or more Wnt inhibitors comprises an inhibitor that acts downstream of the β-catenin destruction complex, and
- obtaining and/or isolating a differentiated liver cell population or a differentiated liver organoid.
Dependencies are inferred from the claim text.