Methods for epicardial differentiation of human pluripotent stem cells
AssigneeWisconsin Alumni Research Foundation
InventorsSean P. Palecek, Xiaoping Bao, Xiaojun Lian
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Abstract
Methods for generating high-yield, high-purity epicardial cells are described. Wnt/β-catenin signaling is first activated in human cardiac progenitor cells, by, for example, inhibiting Gsk-3 to induce differentiation into epicardial cells. Methods for long-term in vitro maintenance of human cardiac progenitor cell-derived epicardial cells and method comprising chemically defined, xeno-free, and albumin-free culture conditions are also provided.
Claims5
A cell culture comprising a chemically defined, albumin-free culture medium comprising an inhibitor of TGFβ signaling and that does not comprise Bone Morphogenetic Protein 4 (BMP4), and a cell population comprising human self-renewing epicardial cells capable of proliferating in vitro for at least 25 cell divisions without differentiation, are not immortalized, and maintain the ability to undergo epithelial-to-mesenchymal transition (EMT), wherein the epicardial cells are derived from a human pluripotent stem cell line in vitro.
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