Methods for producing (6S,15S)-3,8,13,18-tetraazaicosane-6,15-diol

A method of producing a salt of Compound 1,

• comprising the steps of:
• (a) reacting a compound of Formula (I),
• wherein each PG is an amino protecting group,
• with a compound of Formula (II),
• wherein X is a leaving group;
• thereby producing a compound of Formula (III),
• (b) reacting the compound of Formula (III) with a reducing agent, thereby producing a compound of Formula (IV),
• (c) reacting the compound of Formula (IV) with an acetylating agent thereby producing a compound of Formula (V),
• (d) reacting the compound of Formula (V) with a reducing agent, thereby producing a compound of Formula (VI),
• and
• (e) deprotecting the compound of Formula (VI), in the presence of an acid, thereby producing the salt of Compound 1.