Systems and methods for biochemical analysis including a base instrument and a removable cartridge

A removable cartridge comprising:

• a cartridge housing having a sample port that opens to an exterior of the cartridge housing and is configured to receive a biological sample, the cartridge housing has an exterior side that is configured to engage a base instrument;
• a rotatable valve disposed within the cartridge housing, the rotatable valve having a fluidic side and a plurality of valve ports that open at the fluidic side, the rotatable valve having at least one flow channel extending between the valve ports, wherein the rotatable valve is rotatable between different rotational positions;
• a microfluidic body having a body side that is slidably coupled to the fluidic side of the rotatable valve, the microfluidic body at least partially defining a fluidic network that includes:
• a sample channel in flow communication with the sample port, the sample channel having a network port that opens to the body side of the microfluidic body;
• a reservoir configured to hold a reagent, the reservoir being in flow communication with a reservoir port that opens to the fluidic side of the microfluidic body;
• and
• a feed channel in flow communication with a reaction chamber of the fluidic network, the feed channel having a feed port that opens to the body side of the microfluidic body;
• wherein the rotatable valve is configured to rotate between first and second rotational positions, the network port being fluidically coupled to the feed port through the rotatable valve when the rotatable valve is in the first rotational position, the reservoir port being fluidically coupled to the feed port through the rotatable valve when the rotatable valve is in the second rotational position.