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US 11,590,493 B2Granted
Assay devices and methods of manufacture
AssigneeBiomerieux
InventorsAaron Wernerehl, Jonathan A. Bruns, Jonathan B. Derrick, Taylor S. Zimmerman, David E. Jones
Life of this patent
Estimating the term…
Abstract
Systems, methods, and apparatuses are provided for self-contained nucleic acid preparation, amplification, and analysis.
Claims25
A method for forming a reaction container, comprising:
- providing a polymeric sheet that comprises an inner planar face and an outer planar face;
- contacting a first inner planar face of the polymeric sheet to a second inner planar face of the polymeric sheet;
- pressing the polymeric sheet between a first forming plate and a second forming plate, wherein at least one of the first or second forming plates has one or more recesses for forming one or more features selected from the group consisting of a reaction chamber, a fluid flow channel, a reagent chamber, or a sample chamber in selected portions of the polymeric sheet;
- propelling a compressed fluid between the inner planar faces of the polymeric sheet while the polymeric sheet is pressed between the forming plates to reform the selected portions of the polymeric sheet into a shape defined by the one or more recesses of the forming plates;
- separating the first forming plate and the second forming plate so the polymeric sheet is no longer pressed between the first and second forming plates;
- and
- removing the reaction container from between the first forming plate and the second forming plate, wherein the provided polymeric sheet comprises a first polymeric sheet and a second polymeric sheet, each polymeric sheet having an inner planar face and an outer planar face, and the method further comprising:
- arranging the first and second polymeric sheets so that the inner planar faces are arranged adjacent to one another,
- performing the pressing step, and
- propelling a compressed fluid between the two polymeric sheets while the two polymeric sheets are pressed between the forming plates to reform the selected portions into a shape defined by the one or more recesses of the forming plates.
7 more elements
A method for forming a reaction container, comprising:
- providing a first polymeric sheet and a second polymeric sheet, wherein the first and second polymeric sheets each comprise an inner planar face and an outer planar face;
- contacting the inner planar face of the first polymeric sheet to the inner planar face of the second polymeric sheet;
- laminating the first polymeric sheet to the second polymeric sheet;
- making one or more seal lines joining the first and second polymeric sheets;
- pressing the first and second polymeric sheets between a first forming plate and a second forming plate, wherein at least one of the first or second forming plates has one or more recesses positioned for reforming the first and second polymeric sheets to form one or more openings in a region defined by the one or more seal lines;
- expanding selected areas of the first and second polymeric sheets into a shape defined by the one or more recesses of the forming plates by blowing a compressed gas between the first and second polymeric sheets while the first and second polymeric sheets are pressed between the forming plates;
- separating the first forming plate and the second forming plate;
- and
- removing the reaction container from between the first forming plate and the second forming plate.
6 more elements
A method for forming a reaction container formed from a first sheet and a second sheet and having a reaction chamber, a reagent reservoir, a channel fluidically connecting the reaction chamber and the reagent reservoir, and one or more dried reagents disposed in the reaction container between the first sheet and the second sheet, the method comprising:
- dispensing one or more liquid reagents onto the first sheet or the second sheet;
- drying the liquid reagents dispensed onto the first sheet or the second sheet;
- laminating the first sheet to the second sheet, wherein the laminating includes heating the first and second sheets and compressing them, and wherein the laminated first and second sheets are reversibly sealed to one another;
- forming one or more seal lines substantially irreversibly bonding the first and second sheets together at the seal lines to define the reaction chamber, the reagent reservoir, and the channel;
- clamping the first and second sheets in a forming die having a first plate and a second plate, wherein the forming die comprises a recess having a shape corresponding to the reagent reservoir;
- propelling a fluid between the first and second sheets while the first and second sheets are clamped in the forming die to reform selected areas of the first and second sheets into the shapes of the recesses;
- and
- removing the reaction container from the forming die.
5 more elements
Dependencies are inferred from the claim text.