Composition and kits for pseudoplastic microgel matrices
AssigneeRochal Technologies, LLC
InventorsJoseph Charles Salamone, Ann Beal Salamone, Katelyn Elizabeth Reilly, Laura Jean Suggs, Eunna Chung, Kelly Xiaoyu-Chen Leung
Life of this patent
Estimating the term…
Abstract
This invention relates generally to water-insoluble but water-swellable and deformable crosslinked PEGylated microgel particles of proteins and protein-based macromolecules that are pseudoplastic (shear thinning) and flow in aqueous media under shear and which can be injected or made to flow, wherein said microgel particles can reform as a cluster of microgel particles when shearing forces are removed. The microgel particles function as a matrix to support cell growth, viability, and proliferation.
Claims17
A composition comprising:
- a plurality of water-insoluble, hydrogel microparticles comprising PEGylated gel microparticles comprising a protein or protein-based biological macromolecule crosslinked with a PEGylating agent;
- where said protein or protein-based biological macromolecule is selected from the group consisting of collagen, gelatin, and combinations thereof;
- wherein the PEGylating agent is α-succinimidyloxyglutaryl-ω-succinimidyloxyglutaryloxypolyoxyethylene (SG-PEG-SG);
- wherein the plurality of water-insoluble, hydrogel microparticles are hydrated by an aqueous medium;
- wherein the plurality of water-insoluble, hydrogel microparticles are obtained by lyophilizing and then grinding monolithic hydrogels;
- wherein the composition includes clusters of the microparticles in the absence of shear, and the composition decreases in viscosity and exhibits viscoelastic solid properties with applied shear;
- and
- wherein a molar ratio of PEGylating, agent to protein and/or protein-based biological macromolecule is from 10:1 to 50:1.
A composition comprising:
- a plurality of water-insoluble, hydrogel microparticles comprising PEGylated gel microparticles comprising a protein or protein-based biological macromolecule crosslinked with a PEGylating agent that is difunctional to poly-functional;
- where said protein or protein-based biological macromolecule is selected from the group consisting of gelatin, collagen, and combinations thereof;
- wherein the plurality of water-insoluble, hydrogel microparticles are hydrated by an aqueous medium;
- wherein the plurality of water-insoluble, hydrogel microparticles are obtained by lyophilizing and then grinding monolithic hydrogels;
- wherein the composition includes clusters of the microparticles in the absence of shear, and the composition decreases in viscosity and exhibits viscoelastic solid and pseudoplastic properties with applied shear;
- and
- wherein a molar ratio of PEGylating agent to protein and/or protein-based biological macromolecule is from 10:1 to 50:1.
Dependencies are inferred from the claim text.